|
KMID : 0648320090150020008
|
|
Journal of The Korean Society of Hypertension
2009 Volume.15 No. 2 p.8 ~ p.19
|
|
|
Comparison of Resveratrol and Red Wine Polyphenolic Compounds in the Inhibitory Effects on Adrenal Catecholamine Release
|
|
Lim Hyo-Jung
Lim Dong-Yoon
|
|
|
Abstract
|
|
|
|
The aim of the present study was to compare resveratrol with polyphenolic compounds of red wine (PCRW) in the inhibitory effects on the CA secretion from the isolated perfused model of the rat adrenal gland. Both of resveratrol (30 μM) and PCRW (60 μg/ml) during perfusion into an adrenal vein for 90 min inhibited the CA secretory responses evoked by ACh (5.32 mM), high K+ (a direct membrane-depolarizer, 56 mM), DMPP (a selective neuronal nicotinic Nn receptor agonist, 100 μM) and McNA-343 (a selective muscarinic M1 receptor agonist, 100 μM) in a time-dependent manner. Also, in the presence of resveratrol or PCRW, the secretory responses of CA evoked by veratridine (an activator of voltage-dependent Na+ channels, 100 μM), Bay-K-8644 (a L-type dihydropyridine Ca2+ channel activator, 10 μM), and cyclopiazonic acid (a cytoplasmic Ca2+-ATPase inhibitor, 10μM) were significantly reduced. In the simultaneous presence of resveratrol or PCRW and L-NAME (an inhibitor of NO synthase, 30 μM), the CA secretory evoked by ACh, high K+, DMPP, McN-A-343, Bay-K-8644 and cyclopiazonic acid were recovered to a considerable extent of the corresponding control secretion compared with the inhibitory effect of resveratrol or PCRW alone. Interestingly, the amount of nitric oxide (NO) released from the adrenal medulla was greatly increased in comparison to its basal release. Taken together, these experimental results demonstrate that resveratrol or PCRW can inhibit the CA secretory responses evoked by stimulation of cholinergic nicotinic receptors, as well as by direct membrane-depolarization in the isolated perfused model of the rat adrenal gland. It seems that this inhibitory effect of resveratrol or PCRW is exerted by inhibiting an influx of both ions through Na+ and Ca2+ channels into the adrenomedullary cells as well as by blocking the release of Ca2+ from the cytoplasmic calcium store, which are mediated at least partly by the increased NO production due to the activation of NO synthase. Based on concentration (μg/ml), it seems that resveratrol (30 μM=6.85 μg/ml)-induced inhibitory effect on CA release is more potent than PCRW (60 μg/ml)-induced effect.
|
|
|
KEYWORD
|
|
|
Resveratrol, catecholamine secretion, adrenal medulla, cholinergic receptors, nitric oxide
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|